ABSTRACT
Object To select the optimum extracting procedure for quercetin from bud of Sophora japonica L. Methods The optimum extracting procedure was selected with the content and extraction effeciency of quercetin from bud of S. japonica by orthogonal test design. Results The optimum extracting procedure was determined with 10 times of 0.05% sodium hydroxide decocting 20 min and 4 times, adjusting pH value to 6, hydrolyzing with 10 times of 4% sulfuric acid for 1 h after precipitated. Conclusion The optimum extracting procedure increased the content and extraction efficiency of quercetin from bud of S. japonica.
ABSTRACT
Objective: To study the effects of four separation and purification methods on total anthraquinone content in Radix et Rhizoma Rhei decoction. Methods: To Compare the purification efficiency of four methods: gelatim precipition, pH adjustment, polyamide adsorption and macropurous resin adsoption, solid content and total anthraquinone in Radix et Rhizoma Rhei decoction were selected as markers. Results: All solid content of Radix et Rhizoma Rhei decoction greatly decreased after purification while the total anthraquinone content was varied from one method to another. The total anthraquinone content retention rate of macroporous adsorption resin Ⅰ and Ⅱ were highest ( 93.21% , 95.63% respectively). Conclusion: Macropurous adsorption resin have a good enriching effect on the separation and purification of total anthraquinone in Radix et Rhizoma Rhei decoction.
ABSTRACT
Objective: To study the effect of water extracting and alcohol extracting process to anthraquinone content in Rhubarb. Methods: Choosing water and different concentration of alcohol as the solvent, the effect of permeating time, the extracting time, the degree of extracting, the amount of solvent and concentration of alcohol to total, conjugated, and deoxidized anthraquinone content were determined by orthogonal test. Results: Using water as the solvent, the degree of extracting is the significant factors to total anthraquinore with ( P